- Amino 1 evu 1 inate Dehydratase in Human Erythroleukemia Cells : An Immunologically Distinct Enzyme
نویسنده
چکیده
Physicochemical and immunologic properties of 6-aminolevulinate (ALA) dehydratase in human K562 erythroleukemia cells were examined. ALA dehydratase activity was found to increase in K562 cells after treatment with butyric acid or selenium oxide. Enzyme activity in untreated K562 cells was comparable to that in normal adult erythrocytes but was increased threeto six-fold in K562 cells treated with 1 .2 mmol/L butyric acid or 0.03 mmol/L selenium oxide. The Michaelis-Menten constant (Km). the inhibitor constant (Ki). and elution profile by diethylaminoethyl
منابع مشابه
Induction of 6-Aminolevulinic Acid Dehydratase in Mouse Friend Virus-Transformed Erythroleukemia Cells During Erythroid Differentiation
The activity of S-aminolevulinic acid (ALA) dehydratase. an enzyme involved in heme biosynthesis. has been shown to increase in Friend virus-transformed murine erythroleukemia (MEL) cells during erythroid differentiation. In this study. the nature of the increase in ALA dehydratase activity in MEL cells was examined using a monospecific antibody directed to the enzyme. A sevenfold increase in A...
متن کاملCell Culture and Induction of Erythroid Differentiation
Physicochemical and immunologic properties of 6-aminolevulinate (ALA) dehydratase in human K562 erythroleukemia cells were examined. ALA dehydratase activity was found to increase in K562 cells after treatment with butyric acid or selenium oxide. Enzyme activity in untreated K562 cells was comparable to that in normal adult erythrocytes but was increased threeto six-fold in K562 cells treated w...
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The activity of delta-aminolevulinic acid (ALA) dehydratase, an enzyme involved in heme biosynthesis, has been shown to increase in Friend virus-transformed murine erythroleukemia (MEL) cells during erythroid differentiation. In this study, the nature of the increase in ALA dehydratase activity in MEL cells was examined using a monospecific antibody directed to the enzyme. A sevenfold increase ...
متن کاملInduction of 6 - Aminolevulinic Acid Dehydratase in Mouse Friend Virus
The activity of S-aminolevulinic acid (ALA) dehydratase. an enzyme involved in heme biosynthesis. has been shown to increase in Friend virus-transformed murine erythroleukemia (MEL) cells during erythroid differentiation. In this study. the nature of the increase in ALA dehydratase activity in MEL cells was examined using a monospecific antibody directed to the enzyme. A sevenfold increase in A...
متن کاملAmino acid sequence of the regulatory-site glyoxylate peptide of biodegradative threonine dehydratase of Escherichia coli.
Incubation of purified Escherichia coli biodegradative threonine dehydratase with glyoxylate resulted in covalent binding of 1 mol of glyoxylate per mol of protein with concomitant loss of enzyme activity. The glyoxylate-binding site was identified as a heptapeptide representing amino acid residues Ser-33-Asn-Tyr-Phe-Ser-Glu-Arg-39 in the protein primary structure. Addition of glyoxylate to a c...
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